Roper MicroMAX User Manual

4411-0039-CE Version 5.A June 27, 2003 *4411-0039-CE*

x MicroMAX System User Manual Version 5.A This page intentionally left blank.

100 MicroMAX System User Manual Version 5.A Baseline Signal Suddenly Changes If you observe a sudden change in the baseline signal, you may have ex

Chapter 10 Troubleshooting 101 Controller Is Not Responding If this message pops up when you click on OK after selecting the "Interface Type&

102 MicroMAX System User Manual Version 5.A Detector loses Temperature Lock The internal temperature of the camera is too high. This might occur if

Chapter 10 Troubleshooting 103 Error occurs at Computer Powerup If an error occurs at boot up, either the interface card is not installed properly

104 MicroMAX System User Manual Version 5.A As shown, there are no conflicts, allowing the three peripheral cards to operate properly. If the PCI S

Chapter 10 Troubleshooting 105 bus arbitration scheme assures that, as long as every PCI card conforms to the PCI guidelines, the on-board memory

106 MicroMAX System User Manual Version 5.A To Remove a Module: 1. Verify that the Controller has been turned OFF. 2. Rotate the two locking scre

107 Appendix A Specifications CCD Arrays Spectral Range Typically: 370-900 for MicroMAX:512BFT and 1024B 400-1000 for MicroMAX:782Y, 782YH

108 MicroMAX System User Manual Version 5.A Temperature Control Setting Mechanism: Temperature is set by the application software. Display: The act

Appendix A Specifications 109 Inputs EXT SYNC: TTL input (BNC) to allow data acquisition to be synchronized with external events. Sense can be posi

11 Chapter 1 Introduction Introduction The Princeton Instruments MicroMAX system is a high-speed, low-noise CCD camera system designed for demandin

110 MicroMAX System User Manual Version 5.A Memory (RAM): Minimum of 32 Mbytes; possibly more depending on experiment design and size of CCD Array.

111 Appendix B Outline Drawings 5.2513.638.75 Figure 51. ST-133A Controller Dimensions

112 MicroMAX System User Manual Version 5.A C-MOUNT(1.00-32 THREAD)4.634.63 2.116.167.05CCD at .690OPTICAL DEPTH0.000MOUNTINGSURFACECOOLING AIR IN

Appendix B Outline Drawings 113 4.63F-MOUNT(2 3/8” - 20 THREADNIKON ADAPTER SHOWN4.63 OPTIONAL TRIPODMOUNT KIT(2550-0312)COOLING AIR OUTLETTYPICA

114 MicroMAX System User Manual Version 5.A NOTES: 1. WEIGHT: 7.0 LBS. 2. POWER DISSIPATION: 90 WATTS AT FULL POWER. 3. AIRFLOW: 30 CFM

Appendix B Outline Drawings 115 NOTES: 1. WEIGHT: 7.0 LBS. 2. POWER DISSIPATION: 90 WATTS AT FULL POWER. 3. AIRFLOW: 30 CFM FAN CAPACITY

116 MicroMAX System User Manual Version 5.A Note: All dimensions are in inches1/4–20 X 3/8MTG. (4 PLACES)ASECTION A AHOUSINGCCD“C” MOUNTADAPTERW/

Appendix B Outline Drawings 117 Note: All dimensions are in inches1/4–20 X 3/8MTG, (4 PLACES)ASECTION A AHOUSINGCCD“C” MOUNTADAPTERQUARTZWINDOW2.4

118 MicroMAX System User Manual Version 5.A Note: All dimensions are in inches1/4–20 X 3/8MTG. (4 PLACES)AHOUSINGCCDVACUUM PORT1.83 (FOCAL PLANE)0.

119 Appendix C Kinetics Mode Introduction Kinetics mode uses the CCD to expose and store a limited number of images in rapid succession. The time i

12 MicroMAX System User Manual Version 5.A Camera Introduction: The function of the camera is to collect very low intensity light and convert the e

120 MicroMAX System User Manual Version 5.A Kinetic Timing Modes Kinetics mode operates with three timing modes: Free Run, Single Trigger, and Mult

Appendix C Kinetics Mode 121 Not SCAN SignalShutteropeningtimeShutterclosingtimeReadoutSTART ACQUIRE command from the software issent automatically

122 MicroMAX System User Manual Version 5.A Not SCAN SignalShutteropeningtimeShutterclosingtimeReadoutSTART ACQUIRE command from the software issen

123 Appendix D Virtual Chip Mode Introduction Virtual Chip mode (a WinView/32 option) is a special fast-acquisition technique that allows frame rat

124 MicroMAX System User Manual Version 5.A The table below shows the minimum exposure time per frame (msec/Frame) and the number of frames per sec

Appendix D Virtual Chip Mode 125 Assumptions: • You are familiar with the WinView/32 software and have read the hardware manuals. • Masking is fo

126 MicroMAX System User Manual Version 5.A • Shutter Type: None • LOGIC OUT Output: Shutter • Readout Mode: Frame Transfer Interface tab card •

Appendix D Virtual Chip Mode 127 11. Click on the Load Default Values button. This enters the default ROI values. These values are: Start pixels o

128 MicroMAX System User Manual Version 5.A Experimental Timing Triggering can be achieved through the software via the Software Trigger timing mod

Appendix D Virtual Chip Mode 129 Load Default Values: Fills in the region of interest X and Y End values based on the Chip X and Y Dimension entrie

Chapter 1 Introduction 13 Controller Data Conversion: The controller accepts the analog data and converts it to digital data using specially design

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131 Appendix E Repumping the Vacuum Introduction Round head cameras are normally shipped with a vacuum level of ~10 mTorr or better to assure pro

132 MicroMAX System User Manual Version 5.A Figure 68. Vacuum Connector Required for Pumping • Phillips screwdriver and a 3/16" nut driver

Appendix E Repumping the Vacuum 133 7. Remove the vacuum system from the Vacuum Connector. While turning the top knob counterclockwise, remove the

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135 Appendix F Spectrometer Adapters Roper Scientific offers a variety of spectrometer adapters for rectangular head (NTE) MicroMAX systems. The mo

136 MicroMAX System User Manual Version 5.A Chromex 250 IS (NTE with or without shutter) 34521 Qty P/N Description 1. 1 2517-0901 Plate, Adapte

Appendix F Spectrometer Adapters 137 ISA HR 320 (NTE with or without shutter) 321554Remove spectrometer coverfor these screws. Qty P/N Descrip

138 MicroMAX System User Manual Version 5.A ISA HR 640 (NTE with or without shutter) 34512 Qty P/N Description 1. 1 2518-0203 Adapter-Female,

Appendix F Spectrometer Adapters 139 JY TRIAX family (NTE without shutter) 21Remove 4 screwsFlanged Spectrometer Mount Qty P/N Description 1.

14 MicroMAX System User Manual Version 5.A Operating System: Windows® 95/ME/2000/XP or Windows NT® Interface: PCI High-Speed Serial I/O card. Comp

140 MicroMAX System User Manual Version 5.A SPEX 270M (NTE with or without shutter) 31254 Qty P/N Description 1. 1 2518-0691 Female Adapter P

Appendix F Spectrometer Adapters 141 SPEX 500M (NTE with or without shutter) 41523 Qty P/N Description 1. 1 2517-0214 Adapter-Female, Spex 50

142 MicroMAX System User Manual Version 5.A SPEX TripleMate (NTE with or without shutter) 1265473 Qty P/N Description 1. 1 2518-0184 Adapter

143 Declarations of Conformity This section of the MicroMAX manual contains the declarations of conformity for MicroMAX systems. MicroMAX systems e

DECLARATION OF CONFORMITY We, ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619 Declare under our sole responsibili

DECLARATION OF CONFORMITY We, ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619 Declare under our sole responsibil

146 DECLARATION OF CONFORMITY We, , ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619, Declare under our sole respo

147 Warranty & Service Limited Warranty: Roper Scientific Analytical Instrumentation Roper Scientific, Inc. (“Roper Scientific,” us,” “we,” “our

148 MicroMAX System User Manual Version 5.A Sealed Chamber Integrity Limited 24 Month Warranty Roper Scientific warrants the sealed chamber integri

Warranty & Service 149 Owner's Manual and Troubleshooting You should read the owner’s manual thoroughly before operating this product. In

Chapter 1 Introduction 15 Appendix B, Outline Drawings includes outline drawings of the MicroMAX cameras and the ST-133A Controller. Appendix C, Ki

150 MicroMAX System User Manual Version 5.A any warranties implied by state law or non-U.S. laws, as hereby limited, the forgoing warranty is exclu

151 Index #-B 64-pin DIN connector 92, 106 A/D converters dual 72 specifications 109 AC power requirements 22 Accessories, alignment of 45 Actua

152 MicroMAX System User Manual Version 5.A Connectors (cont.) ST-133A, Serial COM 94 ST-133A, TTL In/Out 87, 94 ST-133A, Video Output 94 Contac

Index 153 Free Run (cont.) timing diagram 50 timing flow-chart 50 Full frame readout 63 Full Speed (Asynchronous) mode 47 Full Speed (Synchrono

154 MicroMAX System User Manual Version 5.A P-R Pan function 36 Parfocality 41 PCI card driver installation 29 PCI serial interface card 21 diag

Index 155 ST-133A Controller (cont.) zero adjustment 94 Synchronous vs Asynchronous flow chart49 T Technical support 150 Temperature control prob

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Roper Scientific 1 of 14 June 17, 2003 E:\Manual s\USB II Inst allatio n\USB 2.0 Addendum to Manual.do c USB 2.0 Interface Addendum Introductio

USB 2.0 Interface Addendum June 17, 2003 2 of 14 Roper Scientific E:\Manuals\ USB 2.0 Ins tallati on\USB Addendum to Manual.doc USB 2.0 Limitat

USB 2.0 Interface Addendum Roper Scientific 3 of 14 June 17, 2003 E:\Manual s\USB 2. 0 Install ation\USB 2 .0 Addendum to Manual.doc Installing

16 MicroMAX System User Manual Version 5.A Grounding and Safety The apparatus described in this manual is of the Class I category as defined in IEC

USB 2.0 Interface Addendum June 17, 2003 4 of 14 Roper Scientific E:\Manuals\ USB 2.0 Ins tallati on\USB Addendum to Manual.doc To Update the O

USB 2.0 Interface Addendum Roper Scientific 5 of 14 June 17, 2003 E:\Manual s\USB 2. 0 Install ation\USB 2 .0 Addendum to Manual.doc To Install

USB 2.0 Interface Addendum June 17, 2003 6 of 14 Roper Scientific E:\Manuals\ USB 2.0 Ins tallati on\USB Addendum to Manual.doc Figure 2. RSC

USB 2.0 Interface Addendum Roper Scientific 7 of 14 June 17, 2003 E:\Manual s\USB 2. 0 Install ation\USB 2 .0 Addendum to Manual.doc USB 2.0 and

USB 2.0 Interface Addendum June 17, 2003 8 of 14 Roper Scientific E:\Manuals\ USB 2.0 Ins tallati on\USB Addendum to Manual.doc TAXI interface

USB 2.0 Interface Addendum Roper Scientific 9 of 14 June 17, 2003 E:\Manual s\USB 2. 0 Install ation\USB 2 .0 Addendum to Manual.doc 5. You sho

USB 2.0 Interface Addendum June 17, 2003 10 of 14 Roper Scientific E:\Manuals\ USB 2.0 Ins tallati on\USB Addendum to Manual.doc 4. When the R

USB 2.0 Interface Addendum Roper Scientific 11 of 14 June 17, 2003 E:\Manual s\USB 2. 0 Install ation\USB 2 .0 Addendum to Manual.doc Figure 9.

USB 2.0 Interface Addendum June 17, 2003 12 of 14 Roper Scientific E:\Manuals\ USB 2.0 Ins tallati on\USB Addendum to Manual.doc 5. Using Notep

USB 2.0 Interface Addendum Roper Scientific 13 of 14 June 17, 2003 E:\Manual s\USB 2. 0 Install ation\USB 2 .0 Addendum to Manual.doc No CCD Nam

Chapter 1 Introduction 17 Cleaning Turn off all power to the equipment and secure all covers before cleaning the units. Otherwise, damage to the e

USB 2.0 Interface Addendum June 17, 2003 14 of 14 Roper Scientific E:\Manuals\ USB 2.0 Ins tallati on\USB Addendum to Manual.doc This page inte

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19 Chapter 2 Installation Overview The list and diagrams below briefly describe the sequence of actions required to hookup your system and prepare

Copyright 2003 Roper Scientific, Inc. 3660 Quakerbridge Rd Trenton, NJ 08619 TEL: 609-587-9797 FAX: 609-587-1970

20 MicroMAX System User Manual Version 5.A Action Reference 11. If using a microscope Xenon or an Hg arc lamp, turn it on before turning on the co

21 Chapter 3 System Setup Unpacking the System During the unpacking, check the system components for possible signs of shipping damage. If there a

22 MicroMAX System User Manual Version 5.A Power Requirements The MicroMAX system can operate from any one of four different nominal line voltages,

Chapter 3 System Setup 23 Mounting the Camera General The MicroMAX camera can be mounted either horizontally or vertically (nose up or nose down).

24 MicroMAX System User Manual Version 5.A press the locking lever on the mount while rotating the lens clockwise until it comes free and can be pu

Chapter 3 System Setup 25 C-Mount For a camera equipped with a C-mount thread, use the standard C-mount adapter supplied by the microscope manufact

26 MicroMAX System User Manual Version 5.A Microscope Type Diagnostic Instruments Bottom Clamp Type Leica DMR L-clamp Leitz All types NLW-clamp

Chapter 3 System Setup 27 1XHRP 100-NIK"L" bottom clamp Figure 6. Bottom Clamp secured to Relay Lens Microscope optics have very high tr

28 MicroMAX System User Manual Version 5.A Installing the Application Software Installation is performed via the WinView/32 installation process, w

Chapter 3 System Setup 29 To Install an ISA Serial Card: Support for ISA Serial boards has been discontinued as of the release of WinView/32 versio

iii Table of Contents Chapter 1 Introduction...11 Introduction..

30 MicroMAX System User Manual Version 5.A Selecting the Shutter Setting The Shutter Setting push switch on the rear of the Controller sets the shu

Chapter 3 System Setup 31 Connecting the Detector-Controller Cable or the Camera Power/Camera Signal Cables Turn the Controller power OFF (OFF = 0,

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33 Chapter 4 Operation Introduction This chapter begins with sections regarding a number of operating considerations such as EMF, vacuum, cooling,

34 MicroMAX System User Manual Version 5.A enclosure of the camera. Fins on the round head camera shell radiate the heat outward to the surrounding

Chapter 4 Operation 35 Imaging Field of View When used for two-dimensional imaging applications, the MicroMAX camera closely imitates a standard 35

36 MicroMAX System User Manual Version 5.A Note: If more than one device is connected to the video output, the last device is the one that should t

Chapter 4 Operation 37 Note: With a 16-bit A/D converter (not a standard option), the composite video output is disabled during data acquisition. I

38 MicroMAX System User Manual Version 5.A Computer110/220TAXI cable(Serial Com)ControllerSerialCameraPwrEXPERIMENT110/220CameraMicroscopeCameraSig

Chapter 4 Operation 39 described in Chapter 3. Be sure to secure both ends of the cable with the cable-connector screws. • Connect a 75 Ω BNC cabl

iv MicroMAX System User Manual Version 5.A Cooling ...

40 MicroMAX System User Manual Version 5.A Detector Temperature (Setup|Detector Temperature…): -15°C for round camera heads or -45°C for rectangul

Chapter 4 Operation 41 Note: If you are using WinView/32 and a video monitor for focusing, select the Video Focus… mode from the Acquisition menu.

42 MicroMAX System User Manual Version 5.A setscrews as shown in Figure 13. To change the focus setting, proceed as follows. o Loosen the setscre

Chapter 4 Operation 43 it is possible to use a broadband source such as tungsten for the alignment. If this is the case, use a wavelength setting o

44 MicroMAX System User Manual Version 5.A • Controller type: ST-133 • Controller version: 3 or higher • Camera type: Select the array installed

Chapter 4 Operation 45 of focus and adjust for optimum while watching a live display on the monitor, followed by rotating the detector and again ad

46 MicroMAX System User Manual Version 5.A Acquiring Data Once optimum focus and aperture have been achieved, you can switch from Focus (or Video F

47 Chapter 5 Timing Modes The Princeton Instruments MicroMAX system has been designed to allow the greatest possible flexibility when synchronizing

48 MicroMAX System User Manual Version 5.A sends the Stop Acquisition command to the camera, instructing it to stop acquisition. Once that frame is

Chapter 5 Timing Modes 49 Background orflatfield on?Computer programscamera with exposureand binning parametersSTARTACQ issued fromcomputer to came

Table of Contents v Timing Modes...

50 MicroMAX System User Manual Version 5.A Shutter opensShutter remains openfor preprogrammedexposure timeSystem waits whileshutter closes Figure 1

Chapter 5 Timing Modes 51 In the PreOpen Shutter mode, on the other hand, shutter operation is only partially synchronized to the experiment. As so

52 MicroMAX System User Manual Version 5.A Also note that, in addition to signal from ambient light, dark charge accumulates during the “wait” time

Chapter 5 Timing Modes 53 Note: If EXT SYNC is still active at the end of the readout, the hardware will interpret this as a second sync pulse, and

54 MicroMAX System User Manual Version 5.A low). More specifically, if the readout time, tR, is greater than the sum of tw1, the time the controll

Chapter 5 Timing Modes 55 ShutterMonitorExternal Sync(negative polarity shown)texptw1tRtccleansacquisitiontRtRtRtRactual exposure timeScan Figure 2

56 MicroMAX System User Manual Version 5.A Non-overlapped: This operation mode is automatically selected by the controlling software when the expos

Chapter 5 Timing Modes 57 counting of the programmed Exposure Time begins when the sync pulse arrives at the Ext Sync connector. The exposure ends

58 MicroMAX System User Manual Version 5.A External Sync(negative polarity shown)ShutterMonitortw1tctexpcleansacquisitiontRtRtRtRactual exposure ti

59 Chapter 6 Exposure and Readout Before each image from the CCD array appears on the computer screen, it must first be read, digitized, and trans

vi MicroMAX System User Manual Version 5.A Inputs ...

60 MicroMAX System User Manual Version 5.A CCD arrays perform three essential functions: photons are transduced to electrons, integrated and stored

Chapter 6 Exposure and Readout 61 Mechanical ShutterAcquire ReadoutClosedOpenExposure timeShutter compensationtexptcScan Figure 28. CCD Exposure w

62 MicroMAX System User Manual Version 5.A Interline For interline CCDs, image smearing may occur due to a small amount of light leaking through to

Chapter 6 Exposure and Readout 63 Array Readout In this section, a simple 6 × 4 pixel CCD is used to demonstrate how charge is shifted and digitize

64 MicroMAX System User Manual Version 5.A digitized is the second column moved into the shift register. The order of shifting in our example is th

Chapter 6 Exposure and Readout 65 Frame Transfer The MicroMAX fully supports frame transfer readout. Operation in this mode is very similar to the

66 MicroMAX System User Manual Version 5.A Interline In this section, a simple 6 × 3 pixel interline CCD is used to demonstrate how charge is shift

Chapter 6 Exposure and Readout 67 4312Empty Readout Register. Exposurehas ended and image is beingtransferred to storage cells.Image has been shift

68 MicroMAX System User Manual Version 5.A Part 4 of Figure 32 illustrates the situation at the end of the readout. Both the imaging and storage ce

Chapter 6 Exposure and Readout 69 where Nx is the smaller dimension of the CCD Ny is the larger dimension of the CCD. tsr is the time needed to

Table of Contents vii Figures Figure 1. MicroMAX Cameras and Controller ...11 Figure

70 MicroMAX System User Manual Version 5.A render the camera photon shot noise limited, the S/N ratio improvement is roughly proportional to the sq

Chapter 6 Exposure and Readout 71 On-Chip Binning for Interline Binning is the process of adding the data from adjacent cells together), and it can

72 MicroMAX System User Manual Version 5.A Software Binning One limitation of hardware binning is that the shift register pixels and the output nod

Chapter 6 Exposure and Readout 73 always be nosier than one designed for optimum noise performance. The MicroMAX camera can be provided with two an

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75 Chapter 7 MicroMAX DIF Camera (Double Image Feature) Introduction This Appendix describes operation of the MicroMAX DIF system. Both the Control

76 MicroMAX System User Manual Version 5.A ESABI: Allows two fast images of equal duration to be acquired. Unlike the IEC and EEC modes, in the ESA

Chapter 7 MicroMAX DIF Camera 77 image. As soon as the first exposure actually begins, returns high, as shown in Figure 35. Thus the positive goin

78 MicroMAX System User Manual Version 5.A Summary of Free Run Timing mode Allows user to capture single images. Requires that the switch, if pres

Chapter 7 MicroMAX DIF Camera 79 EXT. SYNC.READY1st Image2nd ImageMechanicalShutter200 ns5 µs5 µs8 ms8 ms Figure 38. Timing Diagram for Typical IE

viii MicroMAX System User Manual Version 5.A Figure 36. Setup using to Trigger an Event... 77

80 MicroMAX System User Manual Version 5.A Example 2: As shown in Figure 41, the signal from the controller can be used to trigger the controller

Chapter 7 MicroMAX DIF Camera 81 supplied by the user is required to initiate the imaging process and control the first image exposure time. The co

82 MicroMAX System User Manual Version 5.A texpEXT. SYNC. (A)READY1st Image2nd ImagetexpttrigNoSignalIntegration Figure 43. ESABI Timing Example:

Chapter 7 MicroMAX DIF Camera 83 controller as well as time-dependent (but constant for a fixed integration time) buildup of dark charge. The backg

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85 Chapter 8 TTL Control Introduction This connector provides 8 TTL lines in, 8 TTL lines out and an input control line. Figure 44 illustrates the

86 MicroMAX System User Manual Version 5.A Table 10 illustrates this coding for decimal values 0 through 7. Obviously this table could easily be ex

Chapter 8 TTL Control 87 Pin # Assignment Pin # Assignment 1 IN 1 14 IN 2 2 IN 3 15 IN 4 3 IN 5 16 IN 6 4 IN 7 17 IN 8 5 GND 18 GN

88 MicroMAX System User Manual Version 5.A • RG/58U coaxial cable. • Shielded Metalized hood (Radio Shack part no 276-1536A). • BNC connector(s)

89 Chapter 9 System Component Descriptions MicroMAX Camera CCD Array: MicroMAX offers a choice of CCD technologies to improve quantum efficiency (Q

Table of Contents ix Table 10. Bit Values with Decimal Equivalents: 1 = High, 0 = Low... 86 Table 11. TTL In/Out Conn

90 MicroMAX System User Manual Version 5.A forced air. CCD temperature is controlled and monitored by via the host computer and the ST-133A Control

Chapter 9 System Component Descriptions 91 drawn into the camera by the internal fan and exhausted through the back panel. The fan is always in op

92 MicroMAX System User Manual Version 5.A ST-133A Controller Electronics: The Model ST-133A is a compact, high performance CCD Camera Controller f

Chapter 9 System Component Descriptions 93 The Analog/Control Module, which should always be located in the left-most slot, provides the following

94 MicroMAX System User Manual Version 5.A The descriptions of the rear panel connectors that follow are keyed to Figure 46. # Feature 1. Temperat

Chapter 9 System Component Descriptions 95 # Feature 12. Camera Pwr: (5 MHz system) Provides the power to operate the camera. The power cable must

96 MicroMAX System User Manual Version 5.A Selecting the wrong shutter setting will result in improper functioning of the shutter and may cause pre

Chapter 9 System Component Descriptions 97 Interface Card PCI Card: The standard interface card plugs-into the host computer's motherboard an

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99 Chapter 10 Troubleshooting Do not attach or remove any cables while the MicroMAX system is powered on. Introduction The following issues have co